GATEWAY Cloning and Expression Vectors
Vector |
Map |
Seq |
Promoter |
Selection |
Tag |
Protease Cleavage Site |
Host |
Origin |
Source |
Description |
| pDEST14 | map | seq | T7 | amp | none | none | BL21 (DE3) | pBR322 | Invitrogen | expression vector |
| pDEST15 | map | seq | T7 | amp | N-GST | none | BL21 (DE3) | pBR322 | Invitrogen | expression vector |
| pDEST17 | map | seq | T7 | amp | N-His | none | BL21 (DE3) | pBR322 | Invitrogen | expression vector |
| pDONR 201 | map | seq | none | amp | none | none | DH5a | Invitrogen | donor vector | |
| pDONR 207 | map | seq | none | gen | none | none | DH5a | Invitrogen | donor vector | |
| pETG-10A | map | seq | T7/lac | amp | H-His | none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b |
| pETG-10K | map | seq | T7/lac | kan | H-His | none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d |
| pETG-20A | map | seq | T7/lac | amp | N-Trx | none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b |
| pETG-20K | map | seq | T7/lac | kan | N-Trx | none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d |
| pETG-30A | map | seq | T7/lac | amp | N-His N-GST |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b |
| pETG-30K | map | seq | T7/lac | kan | N-His N-GST |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d |
| pETG-40A | map | seq | T7/lac | amp | N-MBP | none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b |
| pETG-40K | map | seq | T7/lac | kan | N-MBP | none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d |
| pETG-41A | map | seq | T7/lac | amp | N-His N-MBP |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b |
| pETG-41K | map | seq | T7/lac | kan | N-His N-MBP |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d |
| pETG-50A | map | seq | T7/lac | amp | N-DsbA N-His |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b leader sequence for export to periplasm |
| pETG-50K | map | seq | T7/lac | kan | N-DsbA N-His |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d leader sequence for export to periplasm |
| pETG-52A | map | seq | T7/lac | amp | N-LL DsbA N-His |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b leaderless (LL) DsbA fusion for cytoplasmic expression |
| pETG-52K | map | seq | T7/lac | kan | N-LL DsbA N-His |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d leaderless (LL) DsbA fusion for cytoplasmic expression |
| pETG-60A | map | seq | T7/lac | amp | N-NusA N-His |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-22b |
| pETG-60K | map | seq | T7/lac | kan | N-NusA N-His |
none | BL21 (DE3) | pBR322 | A. Geerlof | expression vector based on pET-24d |