Cloning and Auxiliary Vectors
Vector |
Map |
Seq |
Promoter |
Selection |
Tag |
Protease Cleavage Site |
Host |
Origin |
Source |
Description |
| pACYC177 | map | seq | kan amp |
none | none | DH5a | p15A | New England Biolabs | cloning vector with p15A origin | |
| pET-24(+) | map | seq | T7/lac | kan | C-His | none | BL21 (DE3) | pBR322 | Novagen | transcription vector NB: no ribosome binding site or translation start codon |
| pJY1 pLysE-ArgU | map | seq | T7 Tc |
cam | none | none | BL21 (DE3) | p15a | Affiniti | same as pJY2 except that more T7-lysozyme is produced due to opposite orientation |
| pJY2 pLysS-ArgU | map | seq | T7 Tc |
cam | none | none | BL21 (DE3) | p15a | Affiniti | encodes three "rare" tRNAs and T7-lysozyme |
| pLysS | map | seq | T5/lac | cam | none | none | BL21 (DE3) | p15a | Qiagen | encodes T7 lysozyme that blocks leaky expression from T7 promoter by inhibibiting T7 RNA polymerase |
| pPCR-Script | map | seq | T7 T3 |
amp | none | none | DH5a | ColE1 | Stratagene | cloning vector |
| pRARE | map | seq | cam | none | none | p15A | Novagen | encodes 10 rare tRNA codons | ||
| pREP4 | map | seq | unknown | kan | none | none | M15 | p15A | Qiagen | leaky expression before induction blocked by high level expression of lac I |
| pSP73 | map | seq | SP6 | amp | none | NA | DH5a | unknown | Promega | cloning and in vitro transcription vector |
| pTA Plus | map | seq | T7 T3 |
amp | none | none | DH5a | ColE1 | A. Geerlof | TA-cloning vector |
| pUBS argU amp | map | seq | amp | none | none | p15A | encodes rare arg codon | |||
| pUBS argU kan | map | seq | kan | none | none | p15A | encodes rare arg codon |