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Bacterial Expression Vectors

List of vectors that we can provide to external users

Vector
Map
Seq
Promoter
Selection
Tag
Protease cleavage site
Host
Origin
Source
Description
pACYC177 map seq   kan
amp		 none none DH5a p15A New England Biolabs cloning vector with p15A origin
pASK75 map seq tet amp Strep unknown BL21 unknown Biometra expression vector
pBAD/His A map seq araBAD amp N-His EK TOP10
LMG194		 pUC Invitrogen expression vector
pBAD/His B map seq araBAD amp N-His EK TOP10
LMG194		 pUC Invitrogen expression vector
pBAD/His C map seq araBAD amp N-His EK TOP10
LMG194		 pUC Invitrogen expression vector
pBAD/MCS map seq araBAD amp none none TOP10
LMG194		 pUC A. Geerlof expression vector
pBADM-11 map seq araBAD amp N-His TEV TOP10
LMG194		 pUC A. Geerlof expression vector
pBADM-20 map seq araBAD amp N-TrxA
N-His		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector
pBADM-20(+) map seq araBAD amp N-TrxA
N-His		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-30 map seq araBAD amp N-His
N-GST		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector
pBADM-30(+) map seq araBAD amp N-His
N-GST		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-41(+) map seq araBAD amp N-His
N-MBP		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-52 map seq araBAD amp N-LL-DsbA
N-His		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector
pBADM-52(+) map seq araBAD amp N-LL-DsbA
N-His		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBADM-60 map seq araBAD amp N-NusA
N-His		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector
pBADM-60(+) map seq araBAD amp N-NusA
N-His		 TEV TOP10
LMG194		 pUC A. Geerlof expression vector; contains two STOP codons in MCS
pBAT4 map seq T7 amp none none BL21 (DE3) pUC M. Hyvönen expression vector
pBAT5 map seq T7 amp none none BL21 (DE3) pUC M. Hyvönen expression vector
pCal-n map seq T7/lac amp N-CBP Thr BL21 (DE3) ColE1 Stratagene expression vector
pET-3a map seq T7 amp none none BL21 (DE3) pBR322 Novagen expression vector
pET-3b map seq T7 amp none none BL21 (DE3) pBR322 Novagen expression vector
pET-3c map seq T7 amp none none BL21 (DE3) pBR322 Novagen expression vector
pET-3d map seq T7 amp none none BL21 (DE3) pBR322 Novagen expression vector formerly pET-8c
pET-12a map seq T7 amp leader sequence none BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pET-14b map seq T7 amp N-His Thr BL21 (DE3) pBR322 Novagen expression vector
pET-15b map seq T7/lac amp N-His Thr BL21 (DE3) pBR322 Novagen expression vector
pET-16b map seq T7/lac amp N-His Xa BL21 (DE3) pBR322 Novagen expression vector
pET-19b map seq T7/lac amp N-His Ent BL21 (DE3) pBR322 Novagen expression vector
pET-20b(+) map seq T7 amp leader sequence
C-His		 none BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pET-21d(+) map seq T7/lac amp C-His none BL21 (DE3) pBR322 Novagen expression vector
pET-22b(+) map seq T7/lac amp leader sequence
C-His		 none BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pET-24d(+) map seq T7/lac kan C-His none BL21 (DE3) pBR322 Novagen expression vector
pET-28a map seq T7/lac kan N-His
C-His		 Thr BL21 (DE3) pBR322 Novagen expression vector
pET-28c map seq T7/lac kan N-His
C-His		 Thr BL21 (DE3) pBR322 Novagen expression vector
pET-32a(+) map seq T7/lac amp N-Trx
N-His
C-His		 Thr
EK		 AD494 (DE3)

Origami (DE3)

 pBR322 Novagen expression vector for disulfide bond containing proteins
pET-32b(+) map seq T7/lac amp N-Trx
N-His
C-His		 Thr
EK		 AD494 (DE3)
Origami (DE3)		 pBR322 Novagen expression vector for disulfide bond containing proteins
pET-32c(+) map seq T7/lac amp N-Trx
N-His
C-His		 Thr
EK		 AD494 (DE3)
Origami (DE3)		 pBR322 Novagen expression vector for disulfide bond containing proteins
pET-39b(+) map seq T7/lac kan N-DsbA
C-His		 Thr
EK		 BL21 (DE3) pBR322 Novagen vector for expression in periplasm
pET-40b(+) map seq T7/lac kan N-DsbC
N-His
C-His		 Thr
EK		 BL21 (DE3) pBR322 Novagen vector for expression in periplasm
pETM-10 map seq T7/lac kan H-His
C-His		 none BL21 (DE3) pBR322 G. Stier contains EFH insert in modified pET-24d
pETM-11 map seq T7/lac kan N-His TEV BL21 (DE3) pBR322 G. Stier contains MAD insert in modified pET-24d
pETM-12 map seq T7/lac kan N-His Thr BL21 (DE3) pBR322 G. Stier contains ABD insert in modified pET-9d
pETM-13 map seq T7/lac kan none none BL21 (DE3) pBR322 G. Stier contains ABD insert in modified pET-24d
pETM-14 map seq T7/lac kan N-His 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-20 map seq T7/lac amp N-Trx
N-His		 TEV BL21 (DE3) pBR322 G. Stier contains RAGE insert in modified pET-32
pETM-21 map seq T7/lac amp N-Trx
N-His		 Thr BL21 (DE3) pBR322 G. Stier contains RAGE insert in modified pET-32
pETM-22 map seq T7/lac kan N-Trx
N-His		 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-30 map seq T7/lac kan N-His
N-GST		 TEV BL21 (DE3) pBR322 G. Stier contains DCOH insert in modified pET-24d
pETM-33 map seq T7/lac kan N-His
N-GST		 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-40 map seq T7/lac kan N-MBP TEV BL21 (DE3) pBR322 G. Stier modified pET-24d; contains two STOP codons in MCS
pETM-41 map seq T7 kan N-His
N-MBP		 TEV BL21 (DE3) pBR322 G. Stier modified pET-9d; contains two STOP codons in MCS
pETM-43 map seq T7/lac kan N-MBP 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-44 map seq T7/lac kan N-His
N-MBP		 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-50 map seq T7/lac kan N-DsbA
N-His		 TEV BL21 (DE3) pBR322 G. Stier contains RAGE insert in modified pET-24d; leader sequence for export to periplasm
pETM-51 map seq T7/lac kan N-DsbA
N-His		 Thr BL21 (DE3) pBR322 G. Stier contains RAGE insert in modified pET-24d; leader sequence for export to periplasm
pETM-52 map seq T7/lac kan N-LL DsbA
N-His		 TEV BL21 (DE3) pBR322 G. Stier contains RAGE insert in modified pET-24d; leaderless (LL) DsbA fusion for cytoplasmic expression
pETM-54 map seq T7/lac kan N-DsbA
N-His		 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; leader sequence for export to periplasm; contains two STOP codons in MCS
pETM-55 map seq T7/lac kan N-LL DsbA
N-His		 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; leaderless (LL) DsbA fusion for cytoplasmic expression; contains two STOP codons in MCS
pETM-60 map seq T7/lac kan N-NusA
N-His		 TEV BL21 (DE3) pBR322 G. Stier contains ABD insert in modified pET-24d
pETM-66 map seq T7/lac kan N-NusA
N-His		 3C BL21 (DE3) pBR322 A. Geerlof modified pET-24d; contains two STOP codons in MCS
pETM-70 map seq T7/lac kan N-CBP TEV BL21 (DE3) pBR322 A. Geerlof modified pET-24d
pETM-80 map seq T7/lac kan N-DsbC
N-His		 TEV BL21 (DE3) pBR322 G. Stier modified pET-24d; leader sequence for export to periplasm
pETM-82 map seq T7 kan N-LL DsbC
N-His		 TEV BL21 (DE3) pBR322 G. Stier modified pET-9d; leaderless (LL) DsbC fusion for cytoplasmic expression
pGAT map seq T7 amp N-His
N-GST		 Thr BL21 (DE3) pUC M. Hyvönen expression vector
pGAT2 map seq T7 amp N-His
N-GST		 Thr BL21 (DE3) pUC M. Hyvönen expression vector
pGEX-3X map seq tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-4T-1 map seq tac amp N-GST Thr BL21 pBR322 Pharmacia expression vector
pGEX-4T-2 map seq tac amp N-GST Thr BL21 pBR322 Pharmacia expression vector
pGEX-4T-3 map seq tac amp N-GST Thr BL21 pBR322 Pharmacia expression vector
pGEX-5X-1 map seq tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-5X-2 map seq tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-5X-3 map seq tac amp N-GST Xa BL21 pBR322 Pharmacia expression vector
pGEX-6P-1 map seq tac amp N-GST Pre BL21 pBR322 Pharmacia expression vector
pGEX-6P-2 map seq tac amp N-GST Pre BL21 pBR322 Pharmacia expression vector
pGEX-6P-3 map seq tac amp N-GST Pre BL21 pBR322 Pharmacia expression vector
pHAT map seq T7lac amp N-His none BL21(DE3) pUC M. Hyvönen expression vector
pHAT2 map seq T7lac amp N-His none BL21 (DE3) pUC M. Hyvönen expression vector
pKK223-3 map seq tac amp none none lacIq containing strains eg. JM105 pBR322 Pharmacia expression vector
pKK233-2 map seq trc amp none none        Pharmacia expression vector
pMal-c2 map seq tac amp N-MBP Xa TB1 or any lacZa complementing strain pBR322 New England Biolabs expression vector
pMal-p2 map seq tac amp N-leader sequence-MBP Xa TB1 or any lacZa complementing strain pBR322 New England Biolabs vector for expression in periplasm
pProEx HTa map seq trc amp N-His TEV BL21
Life Technologies expression vector
pProEx HTb map seq trc amp N-His TEV BL21
Life Technologies expression vector
pProEx HTc map seq trc amp N-His TEV BL21
Life Technologies expression vector
pQE-16 map seq T5/lac amp N-DHFR

C-His

 none M15 [pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-30 map seq T5/lac amp N-His none M15 [pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-31 map seq T5/lac amp N-His none M15 [pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-32 map seq T5/lac amp N-His none M15 [pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-60 map seq T5/lac amp C-His none M15 [pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-70 map seq T5/lac amp C-His none M15 [pREP4]; SG13009 [pREP4] ColE1 Qiagen expression vector
pQE-80L map seq T5/lac amp N-His none M15 ColE1 Qiagen expression vector also expressing lac I
pQE-81L map seq T5/lac amp N-His none M15 ColE1 Qiagen expression vector also expressing lac I
pQE-82L map seq T5/lac amp N-His none M15 ColE1 Qiagen expression vector also expressing lac I
pRSET A map seq T7 amp N-His EK BL21 (DE3) pLysS ColE1 Invitrogen expression vector
pRSET B map seq T7 amp N-His EK BL21 (DE3) pLysS ColE1 Invitrogen expression vector
pRSET C map seq T7 amp N-His EK BL21 (DE3) pLysS ColE1 Invitrogen expression vector
pTrcHis2 A map seq trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pTrcHis2 B map seq trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pTrcHis2 C map seq trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pTrcHis2/LacZ map seq trc/lac amp C-His none BL21 pUC Invitrogen expression vector
pZA31-Luc map seq PLtetO-1 cam none none DH5aZ1 p15A H. Bujard contains luciferase gene
pZE12-Luc map seq PLlacO-1 amp none none DH5aZ1 ColE1 H. Bujard contains luciferase gene
pZE21-MCS-1 map seq PLtetO-1 kan none none DH5aZ1 ColE1 H. Bujard high copy number expression vector
pZS*24-MCS-1 map seq Plac/ara-1 kan none none DH5aZ1 pSC*101 H. Bujard low copy number expression vector