Services and Charges
Projects
Service charges for the insect cell culture facility
Use of equipment
Materials
Now available: Broad range Protein Molecular Weight Marker (pdf)
Projects
| Small scale screen of expression levels in E. coli | The expression of one of more constructs will be screened at up to 12 different conditions | 120 Euro/screen |
| Large scale protein production | Production of a protein from max. 6 liter of E. coli culture | 120 Euro/screen |
| Protein purification from inclusion bodies | 120 Euro/protein | |
| Preparation of injection material | 6 injections per rabbit | 300 Euro/antigen |
| Preparation of serum from bleeds | 30 Euro/bleed | Coupling of antigen to CNBr-activated resin | 60 Euro/ml resin |
| Use of cell culture facility | Use of consumables provided by the Facility | 8 Euro/visit |
Service charges for the insect cell culture facility
Work |
Time scale |
Price |
| Transposition of DNA into DH10Bac, bacmid prep and PCR confirmation of insertion. | 1 week | 100 Euro (maximum of 4 constructs in price) |
| Transfection of verified bacmid into Sf9 cells and small scale test expression. | 1.5 weeks | 150 Euro (maximum of 8 constructs in price) |
| Generation of P2/P3 virus and small scale test expression. | 2 weeks | 50 Euro / 50-100 ml |
| Titering of P2 or P3 virus | 1.5 week | 100 Euro |
| Large scale expression in Sf9 cells (Ex-Cell 420 medium, serum free) | 1-2 weeks | 80 Euro / 250 ml culture |
| Large scale expression in High 5 cells (TNMH medium, includes 10% FCS) | 1-2 weeks | 100 Euro / 250 ml culture i.e. 10x 175cm2 |
| Large scale expression in TniHi 5 cells (Ex-Cell 405 medium, serum free) | 1-2 weeks | 100 Euro / 250ml culture |
Use of equipment
| Analytical ultracentrifuge (AUC) | 120 Euro/run |
| Isothermal calorimeter (ITC) | 120 Euro/run |
| Protein purification from inclusion bodies | to be determined |
Materials
| Bacterial vectors | no charge |
| Insect vectors | no charge |
| E. coli strains | no charge |
| Cell lines (50 ml) | 35 Euro |
| His-tagged TEV protease | 20 Euro/mg |
| His-tagged 3C protease | 20 Euro/mg |
| GST-tagged 3C protease | 20 Euro/mg |
| T7 RNA polymerase | 10 Euro/mg |
| Taq polymerase | 20 Euro/mg |
| Pfu polymerase | 20 Euro/mg |
| Additional enzymes (produced rarely on demand) | |
| Murine LIF | 100 Euro/mg |
| Cre recombinase | 100 Euro/mg |
| Protein Molecular Weight Marker | 8Euro/300µl |