The Protein Expression and Purification Core Facility
In recent years the use of recombinant proteins in scientific research has increased greatly, as has the wealth of techniques and products used for their amplification and purification. In the frame of the Core Facilities, EMBL provides a specialised facility (Protein Expression and Purification Core Facility) to help researchers to successfully produce their recombinant proteins and to offer technical assistance and training.
Our main tasks are:
- Providing materials and know-how:
- Providing information on our website describing the process of cloning, and of protein expression, purification and characterisation.
- Assisting in the biophysical analysis of the proteins.
- Establishing and maintaining collections of expression vectors and host cells for prokaryotic as well as eukaryotic expression systems.
- Providing vectors, host strains (cells), and proteins (such as proteases and polymerases).
- Developing and testing new vectors and protocols for the expression systems we support.
- Providing information and assistance.
- Running and maintaining equipment for cultivation of bacteria, yeast and insect cells, and for protein purification and characterisation.
- Producing proteins for the different research groups.
- Keeping track of the latest development in protein expression and purification and to try out new (commercial) products and systems.
If you have any questions or comments on the content of our website please feel free to contact us by email ( pepcore@embl.de ).
For vector requests, please download our Material Transfer Agreement {MTA-link} and send it to us with your signature and the vectors that you would like to have. Please note that we can only provide vectors that were created or modified by EMBL researchers. For commercial vectors, please contact the company that is indicated in our vector tables.