Choice of domains
	Choice of expression system
>	Choice of vector
>  E.coli Vectors Baculovirus Vectors Pichia pastoris Vectors
	PCR Strategy
	Cloning Methods
	Transformation of competent E.coli cells with plasmid DNA

Escherichia coli Vectors - EMBL Vectors

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List of all our E.coli vectors

Vector	PDF	Sequence Text	GeneBank	Promotor	Marker	Tags	Protease cleavage site	Origin	Author
pBAD/MCS	map	seq	gb	araBAD	Amp	none	none	pUC	A. Geerlof
pBADM-11	map	seq	gb	araBAD	Amp	N-His	TEV	pUC	A. Geerlof
pBADM-20	map	seq	gb	araBAD	Amp	N-TrxA N-His	TEV	pUC	A. Geerlof
pBADM-20(+)	map	seq	gb	araBAD	Amp	N-TrxA N-His	TEV	pUC	A. Geerlof
pBADM-30	map	seq	gb	araBAD	Amp	N-His N-GST	TEV	pUC	A. Geerlof
pBADM-30(+)	map	seq	gb	araBAD	Amp	N-His N-GST	TEV	pUC	A. Geerlof
pBADM-40(+)	map	seq	gb	araBAD	Amp	N-MBP	TEV	pUC	A. Geerlof
pBADM-41(+)	map	seq	gb	araBAD	Amp	N-His N-MBP	TEV	pUC	A. Geerlof
pBADM-52	map	seq	gb	araBAD	Amp	N-leaderless-DsbA N-His	TEV	pUC	A. Geerlof
pBADM-52(+)	map	seq	gb	araBAD	Amp	N-leaderless-DsbA N-His	TEV	pUC	A. Geerlof
pBADM-60	map	seq	gb	araBAD	Amp	N-NusA N-His	TEV	pUC	A. Geerlof
pBADM-60(+)	map	seq	gb	araBAD	Amp	N-NusA N-His	TEV	pUC	A. Geerlof
pBAT4	map	seq	gb	T7	Amp	none	none	pUC	M. Hyvönen
pBAT5	map	seq	gb	T7	Amp	none	none	pUC	M. Hyvönen
pETM-10	map	seq	gb	T7-lac	Kan	N-His C-His	none	pBR322	G. Stier
pETM-11	map	seq	gb	T7-lac	Kan	N-His C-His	TEV	pBR322	G. Stier
pETM-12	map	seq	gb	T7	Kan	N-His	Thr	pBR322	G. Stier
pETM-13	map	seq	gb	T7-lac	Kan	none	none	pBR322	G. Stier
pETM-20	map	seq	gb	T7-lac	Amp	N-ThxA N-His C-His	TEV	pBR322	G. Stier
pETM-21	map	seq	gb	T7-lac	Amp	N-TrxA C-His	Thr	pBR322	G. Stier
pETM-30	map	seq	gb	T7-lac	Kan	N-His N-GST C-His	TEV	pBR322	G. Stier
pETM-40	map	seq	gb	T7	Kan	N-MBP	TEV	pBR322	G. Stier
pETM-41	map	seq	gb	T7	Kan	N-His N-MBP	TEV	pBR322	G. Stier
pETM-50	map	seq	gb	T7-lac	Kan	(signal sequence) N-DsbA N-His C-His	TEV	pBR322	G. Stier
pETM-51	map	seq	gb	T7-lac	Kan	(signal sequence) N-DsbA N-His C-His	Thr	pBR322	G. Stier
pETM-52	map	seq	gb	T7-lac	Kan	N-DsbA N-His C-His	TEV	pBR322	G. Stier
pETM-60	map	seq	gb	T7-lac	Kan	N-NusA N-His C-His	TEV	pBR322	G. Stier
pETM-70	map	seq	gb	T7-lac	Kan	N-CBP C-His	TEV	pBR322	A. Geerlof
pETM-80	map	seq	gb	T7-lac	Kan	(signal sequence) N-DsbC N-His C-His	TEV	pBR322	G. Stier
pETM-82	map	seq	gb	T7	Kan	N-DsbC N-His C-His	TEV	pBR322	G. Stier
pGAT	map	seq	gb	T7	Amp	N-His N-GST	Thr	pUC	M. Hyvönen
pGAT2	map	seq	gb	T7	Amp	N-His N-GST	Thr	pUC	M. Hyvönen
pHAT	map	seq	gb	T7-lac	Amp	N-His	none	pUC	M. Hyvönen
pHAT2	map	seq	gb	T7-lac	Amp	N-His	none	pUC	M. Hyvönen